HPLC assay for serum glycated albumin
نویسندگان
چکیده
منابع مشابه
An enzyme-linked immunosorbent assay for glycated albumin.
evidence that oligosaccharide residues are directly involved in C I q binding o r receptor recognition. Instead, it is believed that the carbohydrate of the immunoglobulin Fc portion plays an important structural role in maintaining the correct conformation of the polypeptide in the C,,2 domain. Our experiments show that the complete elimination of the carbohydrate in the C,,2 domain has a smal...
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In this issue of Journal of Diabetes Science and Technology, Kohzuma and colleagues describe a method for measuring glycated amino acids in albumin from serum samples. This method may be useful as an alternative to hemoglobin A1c in monitoring patients with diabetes in certain situations, e.g., diabetes patients with chronic renal failure. Because there are drawbacks of each analyte for measuri...
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Microalbuminuria is a marker of diabetic nephropathy and cardiovascular risk. Immunoassays underestimate the amount of intact albumin present in urine. The purpose of this study was to evaluate a new urinary albumin assay that uses size exclusion high-performance liquid chromatography (HPLC). We determined the limit of detection, linearity, imprecision, a comparison with an immunoturbidimetric ...
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In the diagnosis of diabetes mellitus, hemoglobin A1c (HbA1c) is sometimes measured to determine the need of an oral glucose tolerance test (OGTT). However, HbA1c does not accurately reflect glycemic status in certain conditions. This study was performed to test the possibility that measurement of serum glycated albumin (GA) better assesses the need for OGTT. From 2006 to 2012, 1559 subjects no...
متن کاملBinding of tolbutamide to glycated human serum albumin.
The presence of elevated levels of glucose in blood during diabetes can lead to the non-enzymatic glycation of serum proteins such as human serum albumin (HSA). This study examined the changes that occur in binding of the sulfonylurea drug tolbutamide to HSA as the level of glycation for this protein was increased. High-performance affinity chromatography was used in this work along with column...
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ژورنال
عنوان ژورنال: Diabetologia
سال: 1992
ISSN: 0012-186X,1432-0428
DOI: 10.1007/bf00400267